volumetric ecg recordings biopac systems mp150  (BIOPAC)

Journal: JCI Insight

Article Title: Atrial AMP-activated protein kinase is critical for prevention of dysregulation of electrical excitability and atrial fibrillation

doi: 10.1172/jci.insight.141213

Figure Lengend Snippet: AMPK expression and the development of atrial fibrillation in AMPK double-KO sarcolipin-Cre Prkaa1 fl/fl Prkaa2 fl/fl (AMPK-dKO) compared with littermate control Prkaa1 fl/fl Prkaa2 fl/fl (CON) mice. ( A ) Representative immunoblots with antibodies recognizing the AMPK α2 subunit, both α1 and α2 AMPK subunits (AMPKα), the downstream AMPK target acetyl-CoA carboxylase (ACC), and the phosphorylated form of ACC (pACC) in the left atria (LA), right atria (RA), and left ventricle. ( B ) Corresponding densitometric quantification of the immunoreactive bands. Values are mean ± SEM of n = 6–8 per group. ** P < 0.01, *** P < 0.001 versus CON by unpaired Student’s t test. ( C ) Representative in vivo ECG (lead II) tracings showing normal sinus rhythm in a CON and atrial fibrillation in an atrial AMPK-dKO mouse at 6 weeks of age. Insets show magnified views that demonstrate highly organized atrial P-wave electrical activity in CON and course fibrillatory waves in AMPK-dKO mice. Scale bars: 100 ms. ( D ) Kaplan-Meier analysis showing the 6-month event-free survival from atrial fibrillation in CON ( n = 18) versus AMPK-dKO mice ( n = 23), P < 0.001. ( E ) Graphs show heart rate variability in representative examples of CON mice in sinus rhythm and AMPK-dKO mice in atrial fibrillation, comparing sequential R-R intervals during 60-second recordings ( n = 533 intervals for CON and n = 454 intervals for AMPK-dKO mice).

Article Snippet: Volumetric ECG recordings (Biopac Systems MP150), as well as perfusion pressure and temperature, were recorded throughout the experiment.

Techniques: Expressing, Control, Western Blot, In Vivo, Activity Assay

Journal: JCI Insight

Article Title: Atrial AMP-activated protein kinase is critical for prevention of dysregulation of electrical excitability and atrial fibrillation

doi: 10.1172/jci.insight.141213

Figure Lengend Snippet: Echocardiogram and CT parameters were analyzed in AMPK double-KO sarcolipin-Cre Prkaa1 fl/fl Prkaa2 fl/fl (AMPK-dKO) and littermate control Prkaa1 fl/fl Prkaa2 fl/fl (CON) mice. ( A ) Serial echocardiographic measurements were performed between 4 and 12 weeks of age; graphs show left atrial (LA) area, left ventricular inner diameter at end-diastole (LVID), LV posterior wall thickness (LVPW), LV ejection fraction (LVEF), and heart rate (HR). Values are mean ± SEM, n = 6–9 per group. ( B ) Serial cardiac in vivo micro-CT imaging with contrast enhancement performed between 4 and 15 weeks of age. In representative examples of hearts of 4- and 15-week-old mice, the left panels show grayscale horizontal long axis CT cardiac images, and the right panels demonstrate color 3D segmentation reconstructions of the hearts. CT image acquisition was gated to the ECG cycle and was segmented into 8 phases. Images from atrial diastole (LV end-systole) are shown to visualize the fully filled atria. ( C ) Quantification of cardiac chamber volumes and ventricular ejection fractions (LVEF and RVEF) in mice imaged serially at 4, 8, and 15 weeks of age. Volumes in the fully filled atria (end of LV systole) and ventricles (end of LV diastole) are shown in the graphs. Values are mean ± SEM of n = 9–11 per group. * P < 0.05, ** P < 0.01 versus matched controls by 2-way ANOVA with Holm-Šidák multiple-comparison test.

Article Snippet: Volumetric ECG recordings (Biopac Systems MP150), as well as perfusion pressure and temperature, were recorded throughout the experiment.

Techniques: Control, In Vivo, Micro-CT, Imaging, Comparison

Journal: Plants

Article Title: Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

doi: 10.3390/plants10010064

Figure Lengend Snippet: ECG parameters (RR, QT, QT corrected, ST intervals and heart rate) in rat isoprenaline-induced myocardial ischemia.

Article Snippet: ECG was recorder in lead DII (right forelimb to left hind limb) with a Biopac MP150 system.

Techniques: Control

Journal: Plants

Article Title: Phytochemical Profile, Antioxidant, Cardioprotective and Nephroprotective Activity of Romanian Chicory Extract

doi: 10.3390/plants10010064

Figure Lengend Snippet: ECG parameters principal component analysis (PCA) results: ( A ) ISO group; ( B ) CHT; (C) CHT 1:1; ( D ) CHT 1:3.

Article Snippet: ECG was recorder in lead DII (right forelimb to left hind limb) with a Biopac MP150 system.

Techniques: